Biotechnology · Molecular Biology · Fermentation

Zihao Wang

Biotechnology undergraduate at Henan University of Technology and visiting student at Westlake University. My hands-on experience spans molecular cloning, cell culture, natural product fermentation, microbial screening, and toxin-induced cellular signaling. After graduation, I will join Southern University of Science and Technology as a Research Assistant.

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90.57/100: Arithmetic mean
9/125: Biotechnology cohort rank
3.92/5.0: University GPA scale
4: Publications

Experience

Education and Research Experience

Upcoming

Southern University of Science and Technology · Research Assistant

After graduation, I will join SUSTech as a Research Assistant and continue developing my experimental skills and project execution experience in life science research.

Nov 2025 - Present

Westlake University · Visiting Student

The Role of Cell Death in Bacterial Infection · Supervisor: Dr. Liang Tao

Performed PDCL gene knockout and complementation using CRISPR-Cas9.
Validated phenotypes through functional assays and statistical analysis.
Investigated toxin-induced signaling pathways involving NADPH, NADP, and ROS.

May 2024 - Oct 2025

Henan University of Technology · Natural Product Fermentation

Supervisor: Dr. Zifu Ni

Designed and led Artemisia argyi fermentation experiments across a 72-hour monitoring period.
Quantified polysaccharides, polyphenols, proteins, and free amino acids.
Optimized tobacco enzymatic treatment systems by temperature, pH, and enzyme concentration.

Nov 2022 - May 2024

Henan University of Technology · Microbial Screening and Molecular Cloning

Supervisors: Dr. Zifu Ni and Yanjie Yi

Collected soil samples from multiple regions and carried out microbial isolation and purification.
Applied PCR, qPCR, DNA/RNA extraction, pET-28a plasmid construction, site-directed mutagenesis, protein purification, and SDS-PAGE verification.

Sep 2022 - Present

Henan University of Technology · BSc in Biotechnology

Arithmetic mean: 90.57/100 · Credit-weighted average: 89.24/100 · GPA: 3.92/5.0 on the university scale · Rank: 9/125 in the Biotechnology cohort. Selected coursework includes Cell Biology, Molecular Biology, Genetics, Cell Engineering, Fermentation Engineering, and Microbiology.

Capabilities

Laboratory Skills and Research Capabilities

Molecular Biology

PCR, qPCR, DNA/RNA extraction, Co-IP, plasmid construction, gene cloning and transformation, site-directed mutagenesis, protein expression and purification, SDS-PAGE, yeast electroporation, and sequence alignment.

Cell Culture

Mammalian cell culture with experience in HeLa cells, including routine maintenance, passaging, cell seeding, and sterile cell handling.

Biochemical and Fermentation Analysis

Enzymatic optimization across temperature, pH, and enzyme concentration; dynamic monitoring of polysaccharides, polyphenols, proteins, amino acids; NADPH/NADP/ROS-related pathway analysis.

Languages

Mandarin Chinese native speaker. English IELTS 5.5.

Publications

Ni, Z., Bi, Y., Wang, Z., Han, Y., Bi, Y., Zhang, L., & Sun, S. (2025). Divergent Conversion Efficiencies of Mycobacterium sp. 191574 for Various Phytosterols and Their Underlying Mechanisms. Biomolecules, 15(11), 1496. DOI
Cai M, Ni Z, Sun Z, Li X, Wang Z, et al. (2025). Research Progress Based on SpyTag/SpyCatcher Self-Assembling Peptides and Their Applications in the Food Field. Food Bioengineering, 4(3), 279-291. DOI
Ni Z, Nie A, Imran M, Sun Z, Zhang B, Wang Z, et al. (2025). Dietary fermented Artemisia argyi enhances growth performance, hepatic antioxidant activity, and intestinal health in Hongyu roosters. Poultry Science, 104(12), 105900. DOI
Yi Y, Liu R, Shang Z, Wang K, Zhang C, Wang Z, et al. (2025). Peppermint Essential Oil For Controlling Aspergillus flavus and Analysis of its Antifungal Action Mode. Current Microbiology, 82(4). DOI